The DNA Clean & Concentrator™-5 product provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.
|Format||Spin Column (capped/uncapped)|
|DNA Recovery||Typically, ≤5 µg total DNA can be eluted with ≥6 µl water or low salt elution buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.|
|DNA Size Limits||50 bp to 23 kb|
|Sample Sources||DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.|
|DNA Purity||Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.|
|Product Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS|
Clean & Concentrated DNA. DNA samples, such as the PCR products shown here, can be efficiently purified and concentrated using the DNA Clean & Concentrator™-5