Utilizat pentru colorarea si vizualizarea acizilor nucleici in gelurile de agaroza sau poliacrylamida, si fotodocùmentarea gelurilor de electroforeza utilizand transiluminatoare UV sau cu LED-uri cu lumina albastra.
Detectie ultrasensibila a acizilor nucleici: de 4x mai sensibil decat Bromura de Etidiu
UNIC: are doua varfuri de emisie: coloreaza ADN-ul in VERDE - emisie 520 nm, si ARN-ul in ROSU - 635 nm.
Alternativa Directa pentru SybrGreen si GelGreen.
Livrat sub forma de solutie de colorant pentru ADN si ARN, in flacoane de 1 ML, cu o concentratie 20.000x.
Se utilizeaza ca solutie diluata, cu o concentratie de 1x in gelurile de agaroza sau polyacrylamida, si cu o concentratie de 0,5x in tampoanele de electroforeza.
Non-carcinogen, alternativa pentru Bromura de Etidiu, mult mai economic decat solutiile de colorare SYBRGREEN si GelGREEN.
Economisiti timp pentru vizualizarea acizilor nucleici in gelurile de electroforeza: nu necesita etape de colorare/decolorare in timpul sau dupa migrarea gelurilor.
StainIN™ GREEN Nucleic Acid Stain is a safe alternative to ethidium bromide.
It is same easy to use, four times as sensitive and much more secure.
Twice as economical as competing green dyes, this novel stain can be also used and disposed with less environmental and health concerns compared to ethidium bromide.
It is a fluorescent dye that allows detection of >0,1 ng of DNA in both agarose and polyacrylamide gels.
It binds to both dsDNA, ssDNA and RNA and emits green fluorescence when bound to DNA and red fluorescence when bound to RNA detectable under the UV or Blue light and documented with same filters as similar green dyes.
Due to much less damage to DNA and blue light visualization, StainIN™ GREEN is ideal for DNA extraction from gels for cloning.
The non-carcinogenicity of the dye has been proved by Ames-test. Both mouse marrow erythrocyte micronucleus and spermatocyte chromosomal aberration tests gave negative mutagenicity results.
STORAGE: Store in the dark at +4°C for at least two years. DISPOSAL: 0,5 X dye solution (electrophoresis buffer) can be disposed with plenty of water directly down the drain, used gels can be discarded in the trash. Consult your safety office to match your local regulations, as they vary and change.
Price | 475,00 RON (preturile sunt fara TVA) |
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Description |
StainIN™ GREEN Nucleic Acid Stain is a safe alternative to ethidium bromide. It is same easy to use, four times as sensitive and much more secure. Twice as economical as competing green dyes, this novel stain can be also used and disposed with less environmental and health concerns compared to ethidium bromide. It is a fluorescent dye that allows detection of >0,1 ng of DNA in both agarose and polyacrylamide gels. It binds to both dsDNA, ssDNA and RNA and emits green fluorescence when bound to DNA and red fluorescence when bound to RNA detectable under the UV or Blue light and documented with same filters as similar green dyes. Due to much less damage to DNA and blue light visualization, StainIN™ GREEN is ideal for DNA extraction from gels for cloning. The non-carcinogenicity of the dye has been proved by Ames-test. Both mouse marrow erythrocyte micronucleus and spermatocyte chromosomal aberration tests gave negative mutagenicity results. STORAGE: Store in the dark at +4°C for at least two years. DISPOSAL: 0,5 X dye solution (electrophoresis buffer) can be disposed with plenty of water directly down the drain, used gels can be discarded in the trash. Consult your safety office to match your local regulations, as they vary and change.
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