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08-15-00001, HOT FIREPol® Probe qPCR Mix Plus (no ROX), 1 ml, 250 rxn
529,49 RON
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry
Cost-effective real-time qPCR master mix for probe-based qPCR assays.
This master mix has been developed for TaqMan® probes but is suitable for other hydrolysis probe types as well.
- suitable for singleplex and duplex assays
- high specificity and sensitivity
- reaction set-up and shipment without dry ice
SKU
SBD_08-15-00001
HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe.
The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity.
HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Applications
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
Properties
Concentration: 5x
Hot-start: yes, initial activation in 12-15 min
Detection type: Probe-based
Reference dye: none
Compatible real-time instruments: cyclers that do not need ROX reference dye.
Mix Components
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3 mM MgCl2
dNTPs: dATP, dCTP, dGTP and dTTP
| Price | 444,95 RON (preturile sunt fara TVA) |
|---|---|
| Description |
HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity. HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Applications
Properties Concentration: 5x Hot-start: yes, initial activation in 12-15 min Detection type: Probe-based Reference dye: none Compatible real-time instruments: cyclers that do not need ROX reference dye. Mix Components HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP
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