Anti-E-Cadherin, mouse monoclonal, 1 ml, Species x-Reactivity: human, monkey, mouse, dog/canine, sheep/ovine, Applications: IHC

ECAD

Cat#: BSH-7516-100 100ul, BSH-7516-1 1ml, BSH-7516-RTU 7ml
Clone: BS38
S/R: human, monkey, mouse
Application: IHC
Tissue control: Liver, breast ductal carcinoma, breast lobular carcinoma (negative)

E-Cadherin is a 120 kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression.Tissue specificity: Non-neural epithelial tissues.

E-CAD stained tissue sections. E-CAD Optibody (Clone: BS38) with 1:250 dilution shows similar membranous staining pattern as the optimal staining criteria of NordiQC. E-CAD is used for differentiation diagnosis of ductal breast carcinoma  between lobular breast carcinoma (should be negatively stainined).