Anti-Vimentin, mouse monoclonal, 0,1 ml, Species x-Reactivity: human, Applications: IHC

Vimentin

Cat#: BSH-7100-100, 100ul,    BSH-7100-1, 1ml

Clonality: Mouse monoclonal antibody

Clone: BS13

Application: IHC-P

S/R: Human, sheep

Control tissues: Appendix, tonsil

Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

FFPE sections of appendix, kidney and tonsil have been stained using Vimentin optibody [BS13], 1:200 with polymer detection (KDB-1009). Note the high intensity stained lymphocytes and macrophages but unstained epithelia of appendix (columnar), tonsil (squamous) and epithelia of proximal tubules. Hue of the DAB has been increased using CuSO4 post enhancement method.		Vimentin - Tonsil Tonsil section has been stained using Vimentin optibody (Clone: BS13) with 1:200 dilution. Cells of the mesenchymal origin stained intensively		Vimentin - Appendix Appendix section has been stained using Vimentin optibody (Clone: BS13) with 1:200 dilution. Cells of the mesenchymal origin stained inintensively