The 5-hmC Glucosyltransferase is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a glucose moiety to yield glucosyl-5-hydroxymethylcytosine, which in turn can be used for sequence-specific, genome-wide, or global 5-hmC detection.
Highly processive enzyme for specific modification of 5-hydroxymethylcytosine (5-hmC) with a glucose moiety.
Ideal for locus specific and global quantification of hydroxymethylated DNA.
DESCRIPTION
The 5-hmC Glucosyltransferase is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a glucose moiety to yield glucosyl-5-hydroxymethylcytosine, which in turn can be used for sequence-specific, genome-wide, or global 5-hmC detection.
TECHNICAL SPECIFICATIONS
Concentration
2 U/µl
Standard Reaction Time
2 hours
Storage
5-hmC Glucosyltransferase is guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.
Unit Definition
One unit (U) is defined as the amount of enzyme needed to protect 1 µg of 5-hmC DNA Standard [D5405-3] from Csp6I restriction enzyme digestion via glucosylation in a reaction incubated at 30°C for 1 hour.
Highly processive enzyme for specific modification of 5-hydroxymethylcytosine (5-hmC) with a glucose moiety.
Ideal for locus specific and global quantification of hydroxymethylated DNA.
DESCRIPTION
The 5-hmC Glucosyltransferase is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a glucose moiety to yield glucosyl-5-hydroxymethylcytosine, which in turn can be used for sequence-specific, genome-wide, or global 5-hmC detection.
TECHNICAL SPECIFICATIONS
Concentration
2 U/µl
Standard Reaction Time
2 hours
Storage
5-hmC Glucosyltransferase is guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.
Unit Definition
One unit (U) is defined as the amount of enzyme needed to protect 1 µg of 5-hmC DNA Standard [D5405-3] from Csp6I restriction enzyme digestion via glucosylation in a reaction incubated at 30°C for 1 hour.