D5310-2,   2X Reference Reaction PreMix

D5310-2, 2X Reference Reaction PreMix

D5312, OneStep Plus qMethyl™ PCR kit

3.630,00 RON

The OneStep PLUS qMethyl PCR Kit provides a simple, straightforward, and bisulfite-free procedure for the quantification of DNA methylation at custom-selected genomic regions.

SKU
ZR_D5312

The OneStep PLUS qMethyl PCR Kit provides a simple, straightforward, and bisulfite-free procedure for the quantification of DNA methylation at custom-selected genomic regions. This bisulfite-free assessment is accomplished by splitting the DNA to be tested into two parts: a “Test Reaction” and a “Reference Reaction”. DNA in the Test Reaction is digested with Methylation Sensitive Restriction Enzymes (MSREs) while DNA in the Reference Reaction is not. The DNA from both reactions is then amplified using Real-Time PCR. The difference in Cycle Threshold (Ct) values between the Reference and Test reactions is used to calculate the percentage of DNA methylation at the selected genomic region.

Technical Specifications

Compatible Thermal Cyclers

Bio-rad CFX-96™, CFX Opus 96™, QuantStudio qPCR Systems, Roche LightCycler 480®, or similar.

Detection Dye

Provided dye is compatible with SYBR Green Filter. Assay is flexible with other dyes and TaqMan probes.

Input

High quality DNA, suitable for use in restriction enzyme digestion.
A260/280 > 1.8, A260/230 > 1.8.
Optimal DNA input is 20 ng in 5 µl.
Range validated 5 ng – 1 µg (in 5 µl).

Processing Time

Optimal 14 hours.

Storage

Store at -20°C for up to 12 months. Avoid repeated freeze-thaw cycles of reagents. Prolong storage at -80°C.
2X Test PreMix and 2X Reference PreMix are light sensitive. Minimize light exposure.

 

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Price 3.000,00 RON (preturile sunt fara TVA)
Description

The OneStep PLUS qMethyl PCR Kit provides a simple, straightforward, and bisulfite-free procedure for the quantification of DNA methylation at custom-selected genomic regions. This bisulfite-free assessment is accomplished by splitting the DNA to be tested into two parts: a “Test Reaction” and a “Reference Reaction”. DNA in the Test Reaction is digested with Methylation Sensitive Restriction Enzymes (MSREs) while DNA in the Reference Reaction is not. The DNA from both reactions is then amplified using Real-Time PCR. The difference in Cycle Threshold (Ct) values between the Reference and Test reactions is used to calculate the percentage of DNA methylation at the selected genomic region.

Technical Specifications

Compatible Thermal Cyclers

Bio-rad CFX-96™, CFX Opus 96™, QuantStudio qPCR Systems, Roche LightCycler 480®, or similar.

Detection Dye

Provided dye is compatible with SYBR Green Filter. Assay is flexible with other dyes and TaqMan probes.

Input

High quality DNA, suitable for use in restriction enzyme digestion.
A260/280 > 1.8, A260/230 > 1.8.
Optimal DNA input is 20 ng in 5 µl.
Range validated 5 ng – 1 µg (in 5 µl).

Processing Time

Optimal 14 hours.

Storage

Store at -20°C for up to 12 months. Avoid repeated freeze-thaw cycles of reagents. Prolong storage at -80°C.
2X Test PreMix and 2X Reference PreMix are light sensitive. Minimize light exposure.