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T3010L, Monarch Genomic DNA Purification Kit - 150 preps
3.177,30 RON
The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis, RNA removal, and purification of intact genomic DNA (gDNA) from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues.
SKU
NEB_T3010L
The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis, RNA removal, and purification of intact genomic DNA (gDNA) from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, bacteria and yeast can be processed with extra steps to enhance lysis in these tough-to-lyse samples. Protocols are also included to enable purification from clinically-relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA. Purified gDNA has high quality metrics, including A260/A280 > 1.8 and A260/A230 > 2.0, high DIN scores and minimal residual RNA. The purified gDNA is suitable for downstream applications such as end-point PCR, qPCR and library prep for NGS. It typically has a peak size of > 50kb, making this kit an excellent choice upstream of long-read sequencing platforms.
Specifications:
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Input: |
Cultured mammalian cells: up to 5 x 106 cells Mammalian whole blood: 100 µl Tissue: up to 25 mg, depending on tissue type Bacteria: up to 2 x 109 Yeast: up to 5 x 107 Saliva: up to 500 µl Buccal swabs Genomic DNA requiring cleanup |
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Binding Capacity: |
30 µg genomic DNA |
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Yield: |
Varies depending on sample type |
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Genomic DNA Size: |
Peak size > 50 kb for most sample types; may be lower for saliva and buccal swabs |
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RNA Content |
< 1% (with included RNase A treatment) |
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Elution Volume |
≥35 µl, but 100 µl is recommended |
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Purity: |
A260/280 ≥ 1.8 |
Validated Sample Types:
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| Price | 2.670,00 RON (preturile sunt fara TVA) | |||||||||||||||||
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| Description |
The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis, RNA removal, and purification of intact genomic DNA (gDNA) from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, bacteria and yeast can be processed with extra steps to enhance lysis in these tough-to-lyse samples. Protocols are also included to enable purification from clinically-relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA. Purified gDNA has high quality metrics, including A260/A280 > 1.8 and A260/A230 > 2.0, high DIN scores and minimal residual RNA. The purified gDNA is suitable for downstream applications such as end-point PCR, qPCR and library prep for NGS. It typically has a peak size of > 50kb, making this kit an excellent choice upstream of long-read sequencing platforms. Specifications:
Validated Sample Types:
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