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T2050S, Monarch RNA Cleanup Kit (500 µg) - 10 preps
431,97 RON
The Monarch RNA Cleanup Kit (500 µg) enables fast and simple purification and concentration of up to 500 µg of RNA from in vitro transcription (IVT) and other enzymatic reactions.
SKU
NEB_T2050S
The Monarch RNA Cleanup Kit (500 µg) enables fast and simple purification and concentration of up to 500 µg of RNA from in vitro transcription (IVT) and other enzymatic reactions.
- Ideal for purification of synthesized RNA following high-yield in vitro transcription reactions (alternative to MEGAclear™)
- Optimized for the cleanup of RNA after enzymatic treatments including DNase I, Proteinase K, labeling and capping
- Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
- Elute in ≥ 50 µl for concentrated RNA 70-100% RNA recovery
- Unique column design prevents buffer carryover and elution of silica particulates
- Simplified workflow with a single wash buffer
- Columns and buffers are available separately
- Purified RNA is ready for use in a wide variety of downstream applications, including microinjection and transfection.
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SPECIFICATIONS |
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RNA Sample Type |
Cleanup of RNA from large-scale in vitro transcription reactions |
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Binding Capacity |
500 µg |
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RNA Size Range |
≥ 25 nt ( ≥ 15 nt with modified protocol) |
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Typical Recovery |
70–100% |
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Elution Volume |
50–100 µl |
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Purity |
A260/280 > 1.8 and A260/230 > 1.8 |
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Protocol Time |
10–15 minutes of spin and incubation time |
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Compatible Downstream Applications |
RNA Labeling, RNAi, Microinjections, RT-PCR, RNA library prep for NGS, transfection |
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APPLICATIONS |
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RNA Cleanup and Concentration (including from the TRIzol aqueous phase) |
RNA purified by other methods can be further purified |
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Enzymatic Reaction Cleanup |
Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting |
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In vitro Transcription Cleanup |
Enzymes and excess NTPs are removed to yield highly pure synthesized RNA |
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RNA Gel Extraction |
Purification of RNA from agarose gels |
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RNA Fractionation |
Fractionation of RNA into small and large RNA pools |
| Price | 363,00 RON (preturile sunt fara TVA) | ||||||||||||||||||||||||||||||
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| Description |
The Monarch RNA Cleanup Kit (500 µg) enables fast and simple purification and concentration of up to 500 µg of RNA from in vitro transcription (IVT) and other enzymatic reactions.
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