T2040S, Monarch RNA Cleanup Kit (50 µg) - 10 preps

373,07 RON

The Monarch RNA Cleanup Kit (50 µg) enables fast and simple purification and concentration of up to 50 µg of RNA from enzymatic reactions.

SKU

NEB_T2040S

The Monarch RNA Cleanup Kit (50 µg) enables fast and simple purification and concentration of up to 50 µg of RNA from enzymatic reactions.

Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT)
Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
Elute in ≥ 20 µl for concentrated RNA
70-100% RNA recovery
Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
Simplified workflow with a single wash buffer
Unique column design prevents buffer carryover and elution of silica particulates
Columns and buffers are available separately
Purified RNA is ready for use in a wide variety of downstream applications, including transfection

RNA Sample Type	Cleanup of previously purified RNA (TRIzol, phenol/ chloroform) and RNA from enzymatic reactions (in vitro transcription reactions, DNase I treatment)
Binding Capacity	50 µg
RNA Size Range	≥ 25 nt ( ≥ 15 nt with modified protocol)
Typical Recovery	70–100%
Elution Volume	20–50 µl
Purity	A_260/280> 1.8 and A_260/230 > 1.8
Protocol Time	5 minutes of spin and incubation time
Compatible Downstream Applications	RT =-PCR, RNA library prep for NGS, Formation of ribonucleoprotein (RNP) complexes for genome editing studies, microinjection, RNA labeling, transfection

APPLICATIONS
RNA Cleanup and Concentration (including from the TRIzol aqueous phase)	RNA purified by other methods can be further purified
Enzymatic Reaction Cleanup	Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting
In vitro Transcription Cleanup	Enzymes and excess NTPs are removed to yield highly pure synthesized RNA
RNA Gel Extraction	Purification of RNA from agarose gels
RNA Fractionation	Fractionation of RNA into small and large RNA pools

Price

313,50 RON (preturile sunt fara TVA)

Description

The Monarch RNA Cleanup Kit (50 µg) enables fast and simple purification and concentration of up to 50 µg of RNA from enzymatic reactions.

Ideal for cleanup and concentration of RNA after enzymatic treatments including DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT)
Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt)
Elute in ≥ 20 µl for concentrated RNA
70-100% RNA recovery
Can be used to purify RNA from the aqueous phase following TRIzol® or similar extractions
Simplified workflow with a single wash buffer
Unique column design prevents buffer carryover and elution of silica particulates
Columns and buffers are available separately
Purified RNA is ready for use in a wide variety of downstream applications, including transfection

RNA Sample Type	Cleanup of previously purified RNA (TRIzol, phenol/ chloroform) and RNA from enzymatic reactions (in vitro transcription reactions, DNase I treatment)
Binding Capacity	50 µg
RNA Size Range	≥ 25 nt ( ≥ 15 nt with modified protocol)
Typical Recovery	70–100%
Elution Volume	20–50 µl
Purity	A_260/280> 1.8 and A_260/230 > 1.8
Protocol Time	5 minutes of spin and incubation time
Compatible Downstream Applications	RT =-PCR, RNA library prep for NGS, Formation of ribonucleoprotein (RNP) complexes for genome editing studies, microinjection, RNA labeling, transfection

APPLICATIONS
RNA Cleanup and Concentration (including from the TRIzol aqueous phase)	RNA purified by other methods can be further purified
Enzymatic Reaction Cleanup	Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting
In vitro Transcription Cleanup	Enzymes and excess NTPs are removed to yield highly pure synthesized RNA
RNA Gel Extraction	Purification of RNA from agarose gels
RNA Fractionation	Fractionation of RNA into small and large RNA pools