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T2010S, Monarch Total RNA Miniprep Kit - 50 preps
2.042,04 RON
The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues.
SKU
NEB_T2010S
The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Cleanup of enzymatic reactions or purification of RNA from TRIzol® -extracted samples is also possible using this kit. Purified RNA has high quality metrics, including A260/280 and A260/230 ratios >1.8, high RIN scores, and minimal residual gDNA. Captured RNA ranges in size from full-length rRNAs down to intact miRNAs. Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, cDNA synthesis, RNA-seq, Northern blot analysis, etc.
Properties:
|
Binding Capacity: |
100 µg RNA |
|
RNA Size: |
≥ 20 nt |
|
Purity: |
A260/280 typically ≥ 2.0, A260/230 usually ≥ 1.8 |
|
Tissue Input: |
up to 20 mg for high yield tissues |
|
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up to 50 mg for low yield tissues |
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Cultured Mammalian Cells and Blood Cells (PBMC's, WBC's) Input: |
up to 1 x 106 |
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Mammalian Whole Blood Input: |
up to 3 ml |
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Bacteria Input: |
up to 1 x 109 |
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Yeast Input: |
up to 5 x 107 |
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Plant Input: |
up to 100 mg |
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Elution Volume: |
30 - 100 μl |
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Yield: |
varies depending on sample type |
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Compatible downstream applications: |
RNA Library prep for NGS, RT-PCR, RT-qPCR, Northern blots |
| Price | 1.716,00 RON (preturile sunt fara TVA) | ||||||||||||||||||||||||||
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| Description |
The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Cleanup of enzymatic reactions or purification of RNA from TRIzol® -extracted samples is also possible using this kit. Purified RNA has high quality metrics, including A260/280 and A260/230 ratios >1.8, high RIN scores, and minimal residual gDNA. Captured RNA ranges in size from full-length rRNAs down to intact miRNAs. Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, cDNA synthesis, RNA-seq, Northern blot analysis, etc. Properties:
|
