S9102S, SNAP-Cell 647SiR - 30 nmol

SNAP-Cell^® 647-SiR is a far-red fluorescent substrate that can be used to label SNAP-tag^® fusion proteins inside living cells, on cell surfaces, or in vitro.

• It is a cell-permeable substrate (also termed SiR-SNAP) based on 6-carboxy-tetramethylsiliconrhodamine
• It is suitable for standard Cy5 filter sets
• It has an excitation maximum at 645 nm and an emission maximum at 661 nm

This package contains 30 nmol of SNAP-Cell 647-SiR substrate, sufficient to make 10 ml of a 3 μM SNAP-tag fusion protein labeling solution.

The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on human O⁶-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice.