S6651L, Chitin Resin - 100 ml

3.049,97 RON

An affinity matrix for the isolation of target proteins fused to an intein-chitin binding domain fusion.

SKU

NEB_S6651L

An affinity matrix for the isolation of target proteins fused to an intein-chitin binding domain fusion.

Strong specific binding for CBD-fusion protein affords highly pure protein of interest from crude lysate in one step
Removal of CBD-tag during elution typically yields highly pure, native protein without the use of a protease
This resin, when used with pTXB1, allows for the isolation of native recombinant proteins possessing a reactive C-terminal thioester that can be used for applications in intein-mediated protein ligation (IPL) and site-specific labeling
Binding capacity: 2 mg maltose-binding protein/mL bed volume released from the resin after cleavage of the maltose-binding-CBD-fusion protein
Resin may be regenerated up to 5 times

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Description	An affinity matrix for the isolation of target proteins fused to an intein-chitin binding domain fusion. Strong specific binding for CBD-fusion protein affords highly pure protein of interest from crude lysate in one step Removal of CBD-tag during elution typically yields highly pure, native protein without the use of a protease This resin, when used with pTXB1, allows for the isolation of native recombinant proteins possessing a reactive C-terminal thioester that can be used for applications in intein-mediated protein ligation (IPL) and site-specific labeling Binding capacity: 2 mg maltose-binding protein/mL bed volume released from the resin after cleavage of the maltose-binding-CBD-fusion protein Resin may be regenerated up to 5 times