S1550S, Magnetic mRNA Isolation Kit - 25 isolations

The New England Biolabs Magnetic mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents. The technology is based on the coupling of Oligo d(T)₂₅ to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour. Oligo d(T)₂₅ Magnetic Beads can be reused up to three times and the researcher has the option of eluting the isolated mRNA or using the bound dT DNA as a primer in a first-strand cDNA reaction.

Features

• Suitable for automated high-throughput applications 
• Eliminates need for phenol or other organic solvents 
• No need to precipitate poly(A)+ transcripts in eluent 
• Obtain intact poly(A)+ RNA in less than one hour 
• Negligible gDNA contamination 
• Reusable beads