R3553L,  BsiWI-HF - 1500 units

R3553L, BsiWI-HF - 1500 units

R3539L,  BbsI-HF - 1500 units

R3539L, BbsI-HF - 1500 units

R3539S, BbsI-HF - 300 units

523,60 RON

A high fidelity restriction endonuclease that recognizes the sequence GAAGAC(N)2^.

SKU
NEB_R3539S

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart™ Buffer). All HF-restriction enzymes come with Gel Loading Dye, Purple (6X). Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

Engineered for improved performance
100% activity in rCutSmart Buffer
Used in Golden Gate Assembly
sgRNA Template Construction for CRISPR/Cas Genome Editing
Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Time-Saver™ qualified for digestion in 5-15 minutes
Reduced star activity
Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Restriction Enzyme Cut Site: GAAGAC(2/6)
Can be used with NEBridge® Ligase Master Mix for Golden Gate Assembly


Product Source
An E. coli strain that carries the cloned and modified BbsI gene from Bacillus brevis.

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Price 440,00 RON (preturile sunt fara TVA)
Description

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart™ Buffer). All HF-restriction enzymes come with Gel Loading Dye, Purple (6X). Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

Engineered for improved performance
100% activity in rCutSmart Buffer
Used in Golden Gate Assembly
sgRNA Template Construction for CRISPR/Cas Genome Editing
Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Time-Saver™ qualified for digestion in 5-15 minutes
Reduced star activity
Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Restriction Enzyme Cut Site: GAAGAC(2/6)
Can be used with NEBridge® Ligase Master Mix for Golden Gate Assembly


Product Source
An E. coli strain that carries the cloned and modified BbsI gene from Bacillus brevis.