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P8100S, Endoproteinase GluC - 50 µg
647,96 RON
Endoproteinase GluC is a serine proteinase which preferentially cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues at a rate 100-300 times slower than at glutamic acid residues.
SKU
NEB_P8100S
Endoproteinase GluC is a serine proteinase which preferentially cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues at a rate 100-300 times slower than at glutamic acid residues.
- Ideal for proteome analysis
- Recombinant enzyme with no detectable non-specific protease contaminating activities
- Free of glycerol and detergents which may interfere with Mass Spectrometry or liquid chromatography methods.
- Supplied in dry format
- Optimal activity and stability for up to 12 months
Product Source
Staphylococcus aureus Protease V8 gene cloned and expressed with histidine-tag in Bacillus subtilis
Reconstitution
Endoproteinase GluC should be reconstituted by the addition of 50-500 µl of high purity water. Finger flick the volume of water in the tube to fully resuspend the enzyme. Rapid autolysis is a function of enzyme concentration; any sample reconstituted in a small volume should be used immediately. To get the most use out the enzyme, resuspend the enzyme in 500 μl H
2O and aliquot 50 ul each in 10 tubes. Freeze the tubes that are not being used immediately at -20°C for up to two weeks or less. Storage at - 80°C will prolong enzyme stability approximately 2-4 additional weeks.
| Price | 544,50 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Endoproteinase GluC is a serine proteinase which preferentially cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues at a rate 100-300 times slower than at glutamic acid residues.
Product SourceStaphylococcus aureus Protease V8 gene cloned and expressed with histidine-tag in Bacillus subtilis ReconstitutionEndoproteinase GluC should be reconstituted by the addition of 50-500 µl of high purity water. Finger flick the volume of water in the tube to fully resuspend the enzyme. Rapid autolysis is a function of enzyme concentration; any sample reconstituted in a small volume should be used immediately. To get the most use out the enzyme, resuspend the enzyme in 500 μl H 2O and aliquot 50 ul each in 10 tubes. Freeze the tubes that are not being used immediately at -20°C for up to two weeks or less. Storage at - 80°C will prolong enzyme stability approximately 2-4 additional weeks. |
