P0741L,  Endo S - 30.000 units

P0741L, Endo S - 30.000 units

P0742L,  Endo D - 7.500 units

P0742L, Endo D - 7.500 units

P0741S, Endo S - 6.000 units

1.452,99 RON

Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG.

SKU
NEB_P0741S

Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG.

  • Active on native IgG
  • Tagged with a chitin binding domain (CBD) for easy removal
  • Recombinant enzyme with no detectable exooglycosidase or other endoglycosidases contaminating activities
  • Glycerol -free for optimal performance in HPLC and mass spectrometry analysis
  • ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
  • Optimal activity and stability for up to 12 months

 

Product Source

Endo S is cloned from Streptococcus pyogenes and overexpressed as a fusion to the chitin binding domain in E. coli.

Specificity

Detailed Specificity
X = protein, peptide, Asparagine, or free glycan, as Endo S does not have a strict peptide requirement for activity. Endo S is active on a substrate with or without core α(1-6)fucosylation as well as a with or without a bisecting N-acetylglucosamine. Triantennary and tetrantennary sialyated or asialo glycans are not a substrate for Endo S.

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Price 1.221,00 RON (preturile sunt fara TVA)
Description

Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG.

  • Active on native IgG
  • Tagged with a chitin binding domain (CBD) for easy removal
  • Recombinant enzyme with no detectable exooglycosidase or other endoglycosidases contaminating activities
  • Glycerol -free for optimal performance in HPLC and mass spectrometry analysis
  • ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
  • Optimal activity and stability for up to 12 months

 

Product Source

Endo S is cloned from Streptococcus pyogenes and overexpressed as a fusion to the chitin binding domain in E. coli.

Specificity

Detailed Specificity
X = protein, peptide, Asparagine, or free glycan, as Endo S does not have a strict peptide requirement for activity. Endo S is active on a substrate with or without core α(1-6)fucosylation as well as a with or without a bisecting N-acetylglucosamine. Triantennary and tetrantennary sialyated or asialo glycans are not a substrate for Endo S.