M0651S,  TelN Protelomerase - 250 units

M0651S, TelN Protelomerase - 250 units

M1202S,  Tte-UvrD Helicase - 50 rxns

M1202S, Tte-UvrD Helicase - 50 rxns

M0654S, EnGen Sau Cas9 - 70 pmol

1.047,20 RON

EnGen® Sau Cas9 from Staphylococcus aureus is an RNA-guided DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA. 

SKU
NEB_M0654T

EnGen® Sau Cas9 from Staphylococcus aureus is an RNA-guided DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA.  Targeting requires a ~100 nucleotide guide RNA (gRNA) complementary to the target site, as well as a 5′ NNGRRT 3′ (N = any, R = A or G) protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence.  DNA cleavage by EnGen Sau Cas9 occurs ~3 bases 5′ of the PAM.  EnGen Sau Cas9 has Simian virus 40 (SV40) T antigen nuclear localization signals (NLS) at both the N- and C-termini of the protein.

 

Product Source

EnGen® Sau Cas9 from Staphylococcus aureus is expressed as a C-terminal 6X His-tagged fusion in E. coli.

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Description

EnGen® Sau Cas9 from Staphylococcus aureus is an RNA-guided DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA.  Targeting requires a ~100 nucleotide guide RNA (gRNA) complementary to the target site, as well as a 5′ NNGRRT 3′ (N = any, R = A or G) protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence.  DNA cleavage by EnGen Sau Cas9 occurs ~3 bases 5′ of the PAM.  EnGen Sau Cas9 has Simian virus 40 (SV40) T antigen nuclear localization signals (NLS) at both the N- and C-termini of the protein.

 

Product Source

EnGen® Sau Cas9 from Staphylococcus aureus is expressed as a C-terminal 6X His-tagged fusion in E. coli.