M0653S, EnGen Lba Cas12a (Cpf1) - 70 pmol

EnGen Lba Cas12a (Cpf1) is a programmable DNA endonuclease guided by a single guide RNA (gRNA). Targeting requires a gRNA complementary to the target site as well as a 5' TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a (Cpf1) occurs ~17 bases 3' of the PAM and leaves 5' overhanging ends.

• T-rich TTTV PAM sequence opens up additional genomic regions for targeting
• Shorter, 40-44 base guide RNA
• Two nuclear localization signals for improved transport to the nucleus
• 5’ overhanging termini on cleavage products
• Active from 16 to 48°C
• Maintains activity at lower temperatures than the Acidaminococcus orthologs, permitting editing in ectothermic organisms such as zebra fish and xenopus
• High concentration liquid format can be used for microinjection, electroporation and lipofection.

Reaction Conditions

1X NEBuffer™ r2.1
Incubate at 37°C

1X NEBuffer™ r2.1
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl₂
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Storage Buffer

500 mM NaCl
20 mM sodium acetate
0.1 mM EDTA
0.1 mM TCEP
50% Glycerol
pH 6 @ 25°C

Heat Inactivation

65°C for 10 minutes