M0597S,  NEBNext Q5U Master Mix - 50 rxns (50µl/vol)

M0597S, NEBNext Q5U Master Mix - 50 rxns (50µl/vol)

M0649S,  Nucleoside Digestion Mix - 50 reactions

M0649S, Nucleoside Digestion Mix - 50 reactions

M0645S, Thermostable FEN1 - 1.600 units

562,87 RON

Thermostable Flap Endonuclease 1, FEN1, catalyzes the cleavage of 5´ DNA flaps from branched double stranded DNA substrates, creating a 5´ phosphate terminus. FEN1 products can be ligated by DNA ligase to create double stranded DNA. In vivo, FEN1 is an essential component of the Okazaki fragment maturation pathway, and also plays a role in base excision repair.

SKU
NEB_M0645S

Thermostable Flap Endonuclease 1, FEN1, catalyzes the cleavage of 5´ DNA flaps from branched double stranded DNA substrates, creating a 5´ phosphate terminus. FEN1 products can be ligated by DNA ligase to create double stranded DNA. In vivo, FEN1 is an essential component of the Okazaki fragment maturation pathway, and also plays a role in base excision repair.

Product Source

An E. coli strain, which carries the cloned Thermococcus 9°N FEN1 gene.

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Description

Thermostable Flap Endonuclease 1, FEN1, catalyzes the cleavage of 5´ DNA flaps from branched double stranded DNA substrates, creating a 5´ phosphate terminus. FEN1 products can be ligated by DNA ligase to create double stranded DNA. In vivo, FEN1 is an essential component of the Okazaki fragment maturation pathway, and also plays a role in base excision repair.

Product Source

An E. coli strain, which carries the cloned Thermococcus 9°N FEN1 gene.