M0541S, NEBNext High-Fidelity 2X PCR Master Mix - 50 rxns (50µl/vol)

NEBNext High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5 DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent.

The NEBNext Ultra II Q5 Master Mix (NEB #M0544) is now available, which further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.

The polymerase component of both master mixes, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has the highest fidelity available (> 280-fold higher than that of Taq DNA Polymerase), resulting in ultra-low error rates.

NEBNext High-Fidelity 2X PCR Master Mix contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification.