M0496L, Hot Start Taq 2X Master Mix - 500 rxns

Hot Start Taq 2X Master Mix is an optimized ready-to-use solution containing Hot Start Taq DNA Polymerase, dNTPs, MgCl₂, KCl and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It is recommended for amplification up to 5 kb.

Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions allowing reactions to be set up at room temperature. The aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Hot Start Taq DNA Polymerase possesses a 5´→ 3´ polymerase activity and a 5´ flap endonuclease activity.

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1