M0486L, OneTaq Quick-Load 2X Master Mix with Standard Buffer - 500 rxns

OneTaq^® Quick-Load^® 2X Master Mix with Standard Buffer is an optimized, ready-to-use blend of Taq and Deep Vent^® DNA Polymerases ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3′→5′ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The convenient quick-load master mix formulation contains dNTPs, MgCl₂, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent^® DNA Polymerase gene from Pyrococcus species GB-D.