M0484S, OneTaq Hot Start 2X Master Mix with Standard Buffer - 100 rxns

OneTaq^® Hot Start 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep Vent^® DNA Polymerases combined with an aptamer-based inhibitor.

This enzyme blend is ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase.

 The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgCl₂ and other buffer components and stabilizers listed below, requiring only the addition of primers and DNA template for robust amplification.

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent^® DNA Polymerase gene from Pyrococcus species GB-D.