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M0366S, mRNA Cap 2'-O-Methyltransferase - 2.000 units
478,38 RON
mRNA Cap 2´-O-Methyltransferase adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure.
Recently, it has been reported that the 2′-O-methylation in the cap-1 structure helps the mRNA evade innate immune response in some cell types in vivo.
mRNA Cap 2´-O-Methyltransferase specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5´ end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme (NEB #M2080 ).
Product Source
A His-tagged engineered variant of E. coli that carries the gene for Vaccinia mRNA Cap 2´-O-Methyltransferase.
SKU
NEB_M0366S
mRNA Cap 2´-O-Methyltransferase adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure.
Recently, it has been reported that the 2′-O-methylation in the cap-1 structure helps the mRNA evade innate immune response in some cell types in vivo.
mRNA Cap 2´-O-Methyltransferase specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5´ end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme (NEB #M2080 ).
Product Source
A His-tagged engineered variant of E. coli that carries the gene for Vaccinia mRNA Cap 2´-O-Methyltransferase.
| Price | 402,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
mRNA Cap 2´-O-Methyltransferase adds a methyl group at the 2´-O position of the first nucleotide adjacent to the cap structure at the 5´ end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure. Recently, it has been reported that the 2′-O-methylation in the cap-1 structure helps the mRNA evade innate immune response in some cell types in vivo. mRNA Cap 2´-O-Methyltransferase specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5´ end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme (NEB #M2080 ). Product Source |
