M0345L, Exonuclease V (RecBCD) - 5.000 units

DNA specific exonuclease that also acts as an endonuclease on single-strand DNA
Initiates at both 5' and 3' termini of linear double-stranded DNA
Cleaves linear double-stranded DNA in both the 3' to 5' and 5' to 3' directions
Requires ATP in the reaction

Exonuclease V is ideal for:

Degradation of contaminating linear DNA in plasmid samples
Removal of residual gDNA after purification of low copy plasmid

Product Information
Exonuclease V, a RecBCD complex from E. coli has several different enzyme activities, including an ATP-dependent single-stranded DNA endonuclease activity, ss- and ds- DNA exonuclease activity. The hydrolysis in each case is bi-directional (from both the 3´ and 5´ ends) and processive, producing oligonucleotides. All Exonuclease V activities have divalent cation requirements. Mg2+ is required for the exonuclease activity, while Ca2+ inhibits the exonuclease activity and allows double-stranded DNA unwinding (helicase activity) without hydrolysis.

Product Source
An E. coli strain containing plasmids for expressing the three subunits of E. coli Exonuclease V: RecB, RecC and RecD.