Cloned from Thermotoga maritima and overexpressed in E. coli.
Thermostable bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities
The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and 5,6-dihydroxythymine, generating an AP site. The AP lyase activity cleaves an AP site via β-elimination, creating a 1-nucleotide gap with 3'-α, β-unsaturated aldehyde and 5'-phosphate termini.
Cloned from Thermotoga maritima and overexpressed in E. coli.
Thermostable bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities
The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and 5,6-dihydroxythymine, generating an AP site. The AP lyase activity cleaves an AP site via β-elimination, creating a 1-nucleotide gap with 3'-α, β-unsaturated aldehyde and 5'-phosphate termini.