M0265S, Exonuclease T - 250 units

Exonuclease T (Exo T), also known as RNase T, is a single-stranded RNA or DNA specific nuclease that requires a free 3´ terminus and removes nucleotides in the 3´→ 5´ direction. Exonuclease T can be used to generate blunt ends from RNA or DNA molecules that have 3´ extensions.

Product Source
Exonuclease T is overexpressed and purified as a C-terminal fusion to maltose-binding protein (MBP). MBP is removed from Exonuclease T by Factor Xa cleavage and Exonuclease T is then purified away from Factor Xa and MBP. Exonuclease T cleaved from MBP has an additional amino acid on the N-terminus and a Phe instead of a Met (i.e., Glu-Phe-Exo T instead of Met-Exo T).

Exonuclease T can be used for:

Removal of 3' overhangs of double-stranded DNA to generate blunt-ends*
Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
Removal of single-stranded primers for nested PCR reactions
Removal of single-stranded DNA, leaving behind double-stranded DNA in the sample

*sequence-dependent