M0265L,  Exonuclease T - 1.250 units

M0265L, Exonuclease T - 1.250 units

M0268S,  Endonuclease III (Nth), recombinant - 1.000 units

M0268S, Endonuclease III (Nth), recombinant - 1.000 units

M0265S, Exonuclease T - 250 units

549,78 RON

Exonuclease T (Exo T), also known as RNase T, is a single-stranded RNA or DNA specific nuclease that requires a free 3´ terminus and removes nucleotides in the 3´→ 5´ direction. Exonuclease T can be used to generate blunt ends from RNA or DNA molecules that have 3´ extensions.

SKU
NEB_M0265S

Exonuclease T (Exo T), also known as RNase T, is a single-stranded RNA or DNA specific nuclease that requires a free 3´ terminus and removes nucleotides in the 3´→ 5´ direction. Exonuclease T can be used to generate blunt ends from RNA or DNA molecules that have 3´ extensions.

Product Source
Exonuclease T is overexpressed and purified as a C-terminal fusion to maltose-binding protein (MBP). MBP is removed from Exonuclease T by Factor Xa cleavage and Exonuclease T is then purified away from Factor Xa and MBP. Exonuclease T cleaved from MBP has an additional amino acid on the N-terminus and a Phe instead of a Met (i.e., Glu-Phe-Exo T instead of Met-Exo T).

Exonuclease T can be used for:

Removal of 3' overhangs of double-stranded DNA to generate blunt-ends*
Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
Removal of single-stranded primers for nested PCR reactions
Removal of single-stranded DNA, leaving behind double-stranded DNA in the sample

*sequence-dependent

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Description

Exonuclease T (Exo T), also known as RNase T, is a single-stranded RNA or DNA specific nuclease that requires a free 3´ terminus and removes nucleotides in the 3´→ 5´ direction. Exonuclease T can be used to generate blunt ends from RNA or DNA molecules that have 3´ extensions.

Product Source
Exonuclease T is overexpressed and purified as a C-terminal fusion to maltose-binding protein (MBP). MBP is removed from Exonuclease T by Factor Xa cleavage and Exonuclease T is then purified away from Factor Xa and MBP. Exonuclease T cleaved from MBP has an additional amino acid on the N-terminus and a Phe instead of a Met (i.e., Glu-Phe-Exo T instead of Met-Exo T).

Exonuclease T can be used for:

Removal of 3' overhangs of double-stranded DNA to generate blunt-ends*
Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
Removal of single-stranded primers for nested PCR reactions
Removal of single-stranded DNA, leaving behind double-stranded DNA in the sample

*sequence-dependent