M0264L, RecJf - 5.000 units

RecJ_f is a single-stranded DNA specific exonuclease that catalyzes the removal of deoxy-nucleotide monophosphates from DNA in the 5' → 3' direction.

RecJ_f is a recombinant fusion protein of RecJ and maltose binding protein (MBP). It has the same enzymatic properties as wild-type RecJ. Fusion to MBP enhances RecJ_f solubility.

When DNA containing a 22 base 5' extension is used as a substrate for RecJ_f, the resulting products are a mixture of DNA fragments that have blunt-ends, 5' extensions (1-5 nucleotides) and recessed 5' ends (1-8 nucleotides). RecJ_f does not require a 5' phosphate.

Product Source

RecJ_f is overexpressed and purified as a C-terminal fusion to maltose-binding protein (MBP). MBP does not affect the catalytic activity of RecJ_f but does enhance RecJ_f solubility.