M0262S, Lambda Exonuclease - 1.000 units

Highlights
Isolated from a recombinant source
Highly processive 5'→3' exonuclease
Supplied in 10X Reaction Buffer

Product Source
A genetic fusion of the E. coli Lambda Exonuclease gene with the gene encoding maltose binding protein (MBP). Following affinity chromatography, Lambda Exonuclease is cleaved from the fusion construct and purified away from MBP.

DNA specific exonuclease
Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 5' to 3' direction
Highly processive degradation of double-stranded DNA from the 5' end
Preferred substrate is 5'-phosphorylated double-stranded DNA although non-phosphorylated substrates are degraded at a greatly reduced rate

Lambda Exonuclease is ideal for conversion of linear double-stranded DNA to single-stranded DNA via preferred activity on 5'-phosphorylated ends