M0236S, T4 Polynucleotide Kinase (PNK) (3' phosphatase minus) - 200 units

Product Information
T4 Polynucleotide Kinase catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of double- and single-stranded DNA and RNA, as well as nucleoside 3´-monophosphates.This modified version exhibits full kinase activity with no 3´ phosphatase activity.

5' phosphorylation of DNA/RNA for subsequent ligation
End labeling DNA or RNA for probes and DNA sequencing
5' phosphorylation of 3' phosphorylated mononucleotide to generate a substrate (pNp) that can be added to the 3' end of DNA or RNA
5' end labeling of 3' phosphorylated oligos

Product Source
An E. coli strain that carries a plasmid encoding the modified T4 Polynucleotide Kinase gene.