M0212L, DNA Polymerase Large Fragment exo- - 1.000 units

Klenow Fragment (3´→ 5´ exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has mutations (D355A, E357A) which abolish the 3´→ 5´ exonuclease activity.

Highlights
Isolated from a recombinant source
Generates probes using random primers
Dideoxy sequencing
Supplied with 10X Reaction Buffer
Moderate strand displacement activity

Product Source
An E. coli strain containing a plasmid with a fragment of the E.coli polA (D355A, E357A) gene starting at codon 324.