E7630S, NEBNext Library Quant Kit for Illumina - 100 rxns

Accurate quantitation of next-generation sequencing (NGS) libraries is essential for maximizing data output and quality from each sequencing run. For Illumina^® sequencing specifically, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence output. qPCR is considered to be the most accurate and effective method of library quantitation, providing considerably higher consistency and reproducibility of quantitation. Further, amplification-based methods quantitate only those molecules that contain both adaptor sequences, thereby providing a more accurate estimate of the concentration of the library molecules that can be sequenced. The NEBNext^® Library Quant Kit has been optimized to provide substantial performance and workflow improvements to qPCR-based library quantitation.

• Be confident in your quant values, with more accurate and reproducible results than other methods and kits.
• Enjoy the flexibility to use either four or six standards (included).
• Simplify your reaction setup with fewer pipetting steps and a single extension time for all libraries.
• Use with all your libraries, regardless of insert size, GC content and preparation method.

Features

• Provides more accurate and reproducible quant values than alternative methods and kits
• Compatible with libraries with a broad range of insert sizes and GC content, made by a variety of methods
• Allows you to choose the optimal standard curve (four or six standards) for your experimental needs
• Supplied with a convenient Library Dilution Buffer
• The NEBNext Library Quant Master Mix requires only the addition of primers
• Utilizes a single extension time for all libraries, regardless of insert size
• Library quant values can be easily calculated using NEB’s online tool, at NEBioCalculator.neb.com
• ROX is included in the kit, for use with qPCR instruments that require a reference dye for normalization