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E7550S, NEBNext Ultra II Directional RNA Second Strand Synthesis Module - 24 reactions
3.127,32 RON
The NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext Directional RNA library preparation workflow. This workflow uses the “dUTP” method for strand-specific library construction, which depends on incorporation of uracil into the second strand cDNA, enabled by the NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix, included in this module.
SKU
NEB_E7550S
The NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext Directional RNA library preparation workflow. This workflow uses the “dUTP” method for strand-specific library construction, which depends on incorporation of uracil into the second strand cDNA, enabled by the NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix, included in this module.
The NEBNext Ultra II Directional RNA Second Strand Synthesis Module is Designed for Use with the Following:
- NEBNext® Ultra™ II RNA First Strand Synthesis Module (NEB #E7771) or NEBNext® RNA First Strand Synthesis Module (NEB #E7525)
- NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB #E7546) or NEBNext® Ultra End Repair/dA-Tailing Module (NEB #E7442)
- NEBNext® Ultra™ II Ligation Module (NEB #E7595) or NEBNext® Ultra Ligation Module (NEB #E7445)
- NEBNext® Ultra™ II Q5® Master Mix (NEB #M0544) or NEBNext® High Fidelity 2X PCR Master Mix (NEB #M0541)
- NEBNext® Singleplex or Multiplex Oligos for Illumina® (NEB #E7350, #E7335, #E7500, #E7710, #E7730, #E7600, or #E6609)
- USER™ Enzyme (NEB #M5505)
| Price | 2.628,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
The NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext Directional RNA library preparation workflow. This workflow uses the “dUTP” method for strand-specific library construction, which depends on incorporation of uracil into the second strand cDNA, enabled by the NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix, included in this module. The NEBNext Ultra II Directional RNA Second Strand Synthesis Module is Designed for Use with the Following:
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