E7125L, NEBNext Enzymatic Methyl-seq Conversion Module - 96 assays

The NEBNext Enzymatic Methyl-seq Conversion Module provides a high-performance enzyme-based alternative to bisulfite conversion for methylome analysis.

In the EM-seq workflow, TET2 oxidizes 5-mC and 5-hmC, providing protection from deamination by APOBEC in the next step. In contrast, unmodified cytosines are deaminated to uracils.  

Features:

• High efficiency conversion
• Minimal DNA damage
• Minimized GC bias
• 5-mC and 5-hmC detection
• Complete kit including library prep reagents also available (NEB #E7120)

For specific detection of 5hmC, the NEBNext Enzymatic E5hmC-seq Conversion Module (NEB #E3365) is now also available.