E6800L, PURExpress In vitro Protein Synthesis Kit - 100 reactions

PURExpress® is a reconstituted protein synthesis system based on the PUREsystem™ (Shimizu et al., 2001) where all necessary components needed for in vitro transcription and translation are purified from E. coli.

•    Defined system with all his-tagged proteins for coupled transcription/translation; Ribosome is not his-tagged
•    T7 RNA Polymerase drives in vitro transcription
•    Minimal nuclease and protease activity for stability of synthesized protein and encoding target
•    Templates can be either plasmid DNA, linear DNA or mRNA
•    Protein of interest can be synthesized and visualized in a few hours
•    Synthesizes various target peptides and proteins
•    Synthesized protein can be co-translationally radiolabeled or fluorescently labeled
•    Protein can be reverse-purified or subject to direct functional analysis
•    Applications include high throughput screening/directed evolution, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity and protein-protein interactions
•    Due to reconstituted nature, several kits are offered where translation factors or macromolecules have been omitted to facilitate specific studies
•    Compatible with the PURExpress Disulfide Bond Enhancer (NEB #E6820)