E6420S, NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina - 24 reactions

The NEBNext^® Single Cell/Low Input RNA Library Prep Kit for Illumina^® uses a template switching method to generate full length cDNAs directly from single cells or 2 pg – 200 ng RNA, followed by conversion to sequence-ready libraries using the Ultra™ II FS workflow. This unique workflow enables generation of the highest yields from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance.

NEBNext Multiplex Oligos (Adaptors & Primers) are not included in the kit and are available separately. 

Features:

• The highest yields of high-quality sequencing libraries from single cells, or 2 pg - 200 ng RNA
• Input can be cultured or primary cells, or total RNA
• Low abundance transcripts are easily detected
• High quality libraries and sequence data are generated for a wide range input amounts and sample types
• Full length transcript coverage
• Consistent transcript detection
• Superior transcript correlation
• Fast, streamlined, automation-friendly workflow, with minimal handling steps and hands-on time
• Single-tube protocol from cell lysis to cDNA
• DNA fragmentation, end repair and dA-tailing reagents in a single enzyme mix, with a single protocol, regardless of GC content