E6285L, NEBNext Fast DNA Fragmentation and Library Prep Set for Ion Torrent - 50 reactions

The NEBNext^® Fast DNA Fragmentation & Library Prep Set for Ion Torrent^® includes a master mix of reagents for enzymatic DNA fragmentation combined with end repair. This enables efficient and tunable DNA shearing and blunting with minimized sample loss before performing adaptor ligation and high-fidelity library amplification. The kit is compatible with input amounts from 10 ng - 1µg, with a workflow time of under 2 hours and ~12 min. hands-on time.

Adaptors and primers for singleplex libraries are supplied in the kits. For multiplexed libraries, the Ion Xpress™ Barcode Adapters from Thermo Fisher Scientific can be used.

If mechanical shearing of DNA is preferred, a version of the kit without enzymatic fragmentation reagents is available.