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Romanian
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E6053S, NEBNext dA-Tailing Module - 20 rxns
913,92 RON
Starting Material: 1-5 μg of of end repaired, blunt DNA (100-1000 bp).
SKU
NEB_E6053S
Starting Material: 1-5 μg of of end repaired, blunt DNA (100-1000 bp).
Protocol
- Mix the following components in a sterile microfuge tube:
|
End Repaired, Blunt DNA |
variable |
|
NEBNext dA-Tailing Reaction Buffer (10X) |
5 μl |
|
Klenow Fragment (3´→ 5´ exo–) |
3 μl |
|
Sterile H2O |
variable |
|
Total volume |
50 μl |
- Incubate in a thermal cycler for 30 minutes at 37°C with the heated lid set to ≥ 45°C.
- Purify DNA sample on one spin column or using AMPure XP beads or SPRIselect beads.
| Price | 768,00 RON (preturile sunt fara TVA) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Description |
Starting Material: 1-5 μg of of end repaired, blunt DNA (100-1000 bp). Protocol
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