E1203S, NEB PCR Cloning Kit (Without Competent Cells) - 20 rxns

1.138,83 RON

Easy cloning of all PCR products, including blunt and TA ends

SKU

NEB_E1203S

Easy cloning of all PCR products, including blunt and TA ends

Updated to allow for in vitro transcription with both SP6 and T7 promoters
BsaI-site removed from the ampicillin-resistant gene (allows for the cloning of Golden Gate Assembly modules)
Fast cloning experiments with 5-minute ligation step
Simplified screening with low/no colony background and no blue/white selection required
Save time by eliminating purification steps
Flanking restriction sites available for easy subcloning, including choice of two single digest options
Provided analysis primers allow for downstream colony PCR screening or sequencing
Ready-to-use kit components include 1 kb control amplicon, linearized cloning vector and single-use competent E. coli
Longer shelf life (12 months), as compared to some commercially available products
Also available with competent cells

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Price	957,00 RON (preturile sunt fara TVA)
Description	Easy cloning of all PCR products, including blunt and TA ends Updated to allow for in vitro transcription with both SP6 and T7 promoters BsaI-site removed from the ampicillin-resistant gene (allows for the cloning of Golden Gate Assembly modules) Fast cloning experiments with 5-minute ligation step Simplified screening with low/no colony background and no blue/white selection required Save time by eliminating purification steps Flanking restriction sites available for easy subcloning, including choice of two single digest options Provided analysis primers allow for downstream colony PCR screening or sequencing Ready-to-use kit components include 1 kb control amplicon, linearized cloning vector and single-use competent E. coli Longer shelf life (12 months), as compared to some commercially available products Also available with competent cells