E1000S, K. lactis Protein Expression Kit

The K. lactis Protein Expression Kit provides an easy method for expressing a gene of interest in the yeast Kluyveromyces lactis. Proteins may be produced intracellularly or secreted using an integrative expression vector (pKLAC2). To achieve protein secretion, a gene of interest is cloned downstream of the K. lactis α-mating factor secretion domain, which is eventually processed in the Golgi and results in secretion of the desired protein.

• Supplied with competent K. lactis cells (NEB# C1001)
• Allows cloning and expression of genes toxic to E. coli
• Rapid high cell density growth results in high yield protein expression 
• Easy-to-use protocols for those inexperienced with yeast systems
• No expensive antibiotics or methanol required
• Attractive commercial sub-licensing