E0554S, Q5 Site-Directed Mutagenesis Kit - 10 rxns

1.479,17 RON

The Q5^® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.

SKU

NEB_E0554S

The Q5^® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.

Non-overlapping primer design ensures robust, exponential amplification, generating a high percentage of desired mutations from a wide range of templates
Intramolecular ligation and transformation into NEB high-efficiency competent cells results in high colony yield
Extremely low error rate of Q5 Hot Start High-Fidelity DNA Polymerase reduces screening time
Hot start polymerase enables room temperature reaction set up
DpnI background reduction permits a wide range of starting template concentrations
Use of standard primers eliminates additional expenses from phosphorylated or purified oligos
Easy-to-use PCR master mix and unique multi-enzyme KLD Mix offer convenience and quality
Rapid and direct treatment step proceeds at room temperature in 5 minutes
Allows the use of any chemically-competent E. coli cells suitable for cloning
Supplied with competent cells
Use NEBaseChanger™ tool to generate primer sequences and an annealing temperature

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Price	1.243,00 RON (preturile sunt fara TVA)
Description	The Q5^® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. Non-overlapping primer design ensures robust, exponential amplification, generating a high percentage of desired mutations from a wide range of templates Intramolecular ligation and transformation into NEB high-efficiency competent cells results in high colony yield Extremely low error rate of Q5 Hot Start High-Fidelity DNA Polymerase reduces screening time Hot start polymerase enables room temperature reaction set up DpnI background reduction permits a wide range of starting template concentrations Use of standard primers eliminates additional expenses from phosphorylated or purified oligos Easy-to-use PCR master mix and unique multi-enzyme KLD Mix offer convenience and quality Rapid and direct treatment step proceeds at room temperature in 5 minutes Allows the use of any chemically-competent E. coli cells suitable for cloning Supplied with competent cells Use NEBaseChanger™ tool to generate primer sequences and an annealing temperature