C3040I, NEB Stable Competent E.coli - 24 transformation reactions (6 tubes)

Chemically competent E. coli cells suitable for high efficiency transformation and isolation of plasmid clones containing repeat elements.

Highlights

• Recommended host strain for cloning genes into retroviral/ lentiviral vectors 
• Recommended for cloning of direct repeats and inverted repeat sequences
• Reduced recombination of cloned DNA (recA1)
• Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
• Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
• Resistance to phage T1 (fhuA)
• Suitable for blue/white screening with vectors capable of alpha-complementation

Transformation Efficiency

1-3 x 10⁹ cfu/µg pUC19 DNA

Genotype

F' proA⁺B⁺ lacI^q ∆(lacZ)M15 zzf::Tn10 (Tet^R)/∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15 e14-  Φ80dlacZ∆M15 recA1 relA1 endA1 nupG rpsL (Str^R) rph spoT1 ∆(mrr-hsdRMS-mcrBC)