B7703S, Blue Loading Buffer Pack - 8 ml

The blue protein loading dye contains one vial of blue loading buffer and one vial of 30X reducing agent.

The combined solution is ideal for protein gel applications.

• 1X Blue Loading Buffer Composition: 62.5 mM Tris-HCl (pH 6.8), 2% (w/v) SDS, 10% glycerol, 0.01% (w/v) bromophenol blue
• 30X Reducing Agent: 1.25 M DTT
• SDS interacts with positively charged amino acids of proteins, thereby disrupting interactions that form protein structures to separate proteins based on size rather than charge or shape 
• DTT is present to help reduce structural disulfide bonds 

Blue Loading Buffer Reagents for SDS-PAGE:

3X SDS Blue Loading Buffer (8 ml):
187.5 mM Tris-HCl, pH 7.0
6% SDS (w/v)
30% glycerol
0.03% bromophenol blue (w/v)

30X Reducing Agent (1 ml):
1.25 M DTT
(Store at -20°C)