50202, Collagen Type I, rat tail, 5 mg/ml, 4 x 5 ml: non-pepsinized

A high-quality collagen type I (bovine or rat tail origin) for cell culture applications (e.g., 3D gels, scaffolds, and coating)

• Provides in vivo-like extracellular matrix (ECM) structures
• Non-pepsinized, native collagen for modelling biological ECM in gel matrices
• Fast gelation facilitates optimal cell distribution in 3D gels

Applications

• 3D cell culture in in vivo-like matrices
• Live cell imaging in a 3D environment
• Creating a 3D scaffold for organoids and spheroids
• Preparation of 3D collagen gels following Application Note 26: "Preparation of Collagen I Gels" (PDF)
• Chemotaxis assays in 3D collagen gels using the µ-Slide Chemotaxis
• Cell migration assays of suspension cells (e.g., leukocytes)
• Coating of cell culture vessels with a thin collagen layer to promote cell attachment
• Cell differentiation studies in a 2D versus 3D environment
• Highly suited for fluorescence microscopy due to low autofluorescence

Technical Features

• High-quality purified protein from rat tendons or bovine hides
• Highly standardized manufacturing process with stringent quality control
• Mild manufacturing process for highest levels of nativity
• Detailed protocol for easy handling in Application Note 26: "Preparation of Collagen I Gels" (PDF)
• Shipping on cool packs for well-defined quality and reproducibility
• Available in 2 concentrations: 5 mg/ml for standard applications and a high-concentrated solution with 10 mg/ml for special applications
• Highest level of natural cross-linking on the market (highest quality grade)
• High viscosity: special pipettes for highly viscous solutions are recommended
• Low autofluorescence