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The LyoPrime WarmStart Fluorescent LAMP/RT-LAMP Mix (with UDG) is offered in a lyophilized format, allowing for ambient/room temperature shipping and storage before use. It is a simple, one-step solution for real-time fluorescent detection of LAMP/RT-LAMP reactions. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers and a strand-displacing DNA polymerase. Featuring a blend of Bst 2.0 WarmStart® DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution, this mix offers improved performance. LAMP Fluorescent Dye at 0.5X concentration is included to enable detection of amplification via real time fluorescence. The inclusion of dUTP and thermolabile UDG in the master mix reduces the possibility of carryover contamination, where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. Thermolabile UDG is completely inactivated at temperatures above 50°C, thereby having no effect on the reaction.
This product is available in 2 x 50 reaction glass vials or a 96-well plate (1 reaction per well). The plate features twelve partially-attached 8-well strip tubes, allowing for use of the entire plate or partial consumption. The yellow shell frame adaptor supports the use of the plate in a variety of common real-time PCR instruments. Both formats can be customized.
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| Price | 2.262,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
The LyoPrime WarmStart Fluorescent LAMP/RT-LAMP Mix (with UDG) is offered in a lyophilized format, allowing for ambient/room temperature shipping and storage before use. It is a simple, one-step solution for real-time fluorescent detection of LAMP/RT-LAMP reactions. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers and a strand-displacing DNA polymerase. Featuring a blend of Bst 2.0 WarmStart® DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution, this mix offers improved performance. LAMP Fluorescent Dye at 0.5X concentration is included to enable detection of amplification via real time fluorescence. The inclusion of dUTP and thermolabile UDG in the master mix reduces the possibility of carryover contamination, where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. Thermolabile UDG is completely inactivated at temperatures above 50°C, thereby having no effect on the reaction. |