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Q5 Blood Direct 2X Master Mix can amplify a wide variety of targets direct from dried blood spots or up to 30% whole human blood, skipping DNA purification. The master mix includes Q5 Hot Start High-Fidelity DNA Polymerase, a thermostable DNA polymerase, with 3′→ 5′ exonuclease activity, fused to a processivity-enhancing Sso7d domain and dNTPs in an optimized buffer with increased resistance to inhibitors in blood, anti-coagulants, and chemicals on filter papers. It is capable of amplifying products up to 7.5 kb from human whole blood cells preserved with sodium EDTA, potassium EDTA, sodium citrate and sodium heparin as well samples stored on common preservative filter papers.
Q5 Hot Start High-Fidelity DNA Polymerase offers robust DNA amplification with low error rates. The addition of an aptamer-based inhibitor allows for room temperature reaction setup.
It is recommended to use NEB’s Tm Calculator to determine the appropriate annealing temperature for each primer pair and polymerase/buffer of interest. Unlike other calculators, the NEB Tm Calculator takes buffer components that affect melting temperatures into consideration when calculating the best annealing temperature. Other online calculators may underestimate the best Q5 annealing temperature.
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| Price | 720,00 RON (preturile sunt fara TVA) |
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| Description |
Q5 Blood Direct 2X Master Mix can amplify a wide variety of targets direct from dried blood spots or up to 30% whole human blood, skipping DNA purification. The master mix includes Q5 Hot Start High-Fidelity DNA Polymerase, a thermostable DNA polymerase, with 3′→ 5′ exonuclease activity, fused to a processivity-enhancing Sso7d domain and dNTPs in an optimized buffer with increased resistance to inhibitors in blood, anti-coagulants, and chemicals on filter papers. It is capable of amplifying products up to 7.5 kb from human whole blood cells preserved with sodium EDTA, potassium EDTA, sodium citrate and sodium heparin as well samples stored on common preservative filter papers. |