5' Phosphate 2,5 µmol scale, 1 modification

The most current application of the 5’-PO4 modified oligonucleotide is the direct ligation to a vector without prior use of T4 polynucleotide kinase. 3’-PO4 and 5’-PO4 oligonucleotides may also be used for either further chemical oligonucleotide modification or as a substrate in DNA repair studies. This phosphorylation permits the labelling of large quantities and is more reproducible than enzymatic procedures.