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31-01-00100, Salini™ UNG Uracil-N-Glycosylase, 100 U
471,24 RON
Fast and robust heat-labile Uracil-N-Glycosylase
- Stable at 25 °C for at least 3 months and at 37 °C 2 weeks
- Heat-labile enzyme (compatible with Sanger sequencing). No reactivation is detected after heat inactivation.
- Fast 30 second reaction time
- Tolerant to common inhibitors
- Reaction set-up and shipment without ice
- Glycerol-free formulation is available
SKU
SBD_31-01-00100
Salini UNG® Uracil-N-Glycosylase is a unique heat-labile enzyme. The protein sequence originates from the bacteria genus Salinivibrio which is frequently found in hypersaline environments. Uracil-N-Glycosylase (UNG) efficiently eliminates uracil from single- or doublestranded DNA by catalyzing the hydrolysis of the N-glycosylic bond and leaving an abasic site. This property is widely used as a part of PCR carryover contamination prevention strategy. Salini UNG® is a genetically modified enzyme including a Stability TAG - Solis BioDyne’s proprietary and patented polypeptide stabilization technology that makes all our proteins extremely stable at room temperature.
Applications
- Widely used to eliminate carryover contamination in PCR and LAMP
- Enhancer of cloning efficiency of PCR products
- Site-directed mutagenesis
- Protein-DNA interaction studies
- Glycosylase-mediated single nucleotide polymorphism detection (GMPD)
- Study of DNA repair and mutation detection
- SNP genotyping
Properties
Concentration: 1 U/µl
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a Salini UNG® Uracil-N-Glycosylase gene.
Storage and dilution buffer: 50% glycerol (v/v), 25 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT and stabilizers.
Recommendations for use
Working concentration: For qPCR application we recommend to use Salini UNG® Uracil-N-Glycosylase at a final concentration of 0.025-0.04 U/μl, for endpoint PCR application at a final concentration of 0.005-0.01 U/μl.
Reaction temperature: If you setup reaction at room temperature, no additional treatment step is required. If you setup reaction on ice add UNG treatment step prior amplification: 30 seconds at 25 °C. Working temperature 25-40 °C.
Inactivation: 5 min at 70 °C.
Mai multe informatii
| Price | 396,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Salini UNG® Uracil-N-Glycosylase is a unique heat-labile enzyme. The protein sequence originates from the bacteria genus Salinivibrio which is frequently found in hypersaline environments. Uracil-N-Glycosylase (UNG) efficiently eliminates uracil from single- or doublestranded DNA by catalyzing the hydrolysis of the N-glycosylic bond and leaving an abasic site. This property is widely used as a part of PCR carryover contamination prevention strategy. Salini UNG® is a genetically modified enzyme including a Stability TAG - Solis BioDyne’s proprietary and patented polypeptide stabilization technology that makes all our proteins extremely stable at room temperature. Applications
Properties Concentration: 1 U/µl Source: Purified from an E.coli strain that carries an overproducing plasmid containing a Salini UNG® Uracil-N-Glycosylase gene. Storage and dilution buffer: 50% glycerol (v/v), 25 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT and stabilizers. Recommendations for use Working concentration: For qPCR application we recommend to use Salini UNG® Uracil-N-Glycosylase at a final concentration of 0.025-0.04 U/μl, for endpoint PCR application at a final concentration of 0.005-0.01 U/μl. Reaction temperature: If you setup reaction at room temperature, no additional treatment step is required. If you setup reaction on ice add UNG treatment step prior amplification: 30 seconds at 25 °C. Working temperature 25-40 °C. Inactivation: 5 min at 70 °C. |