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28-21-00001-5, SolisFAST® Probe qPCR Mix with UNG (no ROX), 5 ml, 1250 rxn
3.353,00 RON
Fast, highly sensitive and reproducible probe-based qPCR solution
Extremely fast and highly sensitive probe-based qPCR Mix that has been optimized for detection and quantification of up to five targets
simultaneously.
- delivers qPCR results 2x faster (in as little as 30 minutes)
- reliable quantification in up to 5-plex assays
- consistent results with low- and high-copy targets
- robust performance
- contains dUTP and UNG to prevent carryover contamination
- tolerant to common PCR inhibitors
SKU
SBD_28-21-00001-5
SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously.
Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start and optimized buffer, this qPCR Mix offers accurate target detection and quantification.
For extra convenience the mix includes dUTP and UNG enzyme that enable UNG treatment to prevent carryover contamination.
This master mix is developed for TaqMan® probes but is also suitable for other hydrolysis probe types.
Applications
- DNA/LNA hydrolysis probe based assays
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
Properties
Concentration: 5x
Hot-start: yes, 3 minutes initial incubation at 95 °C
Cycling speed: fast or ultra-fast
Detection type: Probe-based
Reference dye: none
Multiplex: up to 5 plexes simultaneously
Compatible real-time instruments: The mix is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler!
Mix Components:
SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 16.5 mM MgCl2 (1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR
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| Price | 2.817,65 RON (preturile sunt fara TVA) |
|---|---|
| Description |
SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously. Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start and optimized buffer, this qPCR Mix offers accurate target detection and quantification. For extra convenience the mix includes dUTP and UNG enzyme that enable UNG treatment to prevent carryover contamination. This master mix is developed for TaqMan® probes but is also suitable for other hydrolysis probe types. Applications
Properties Concentration: 5x Hot-start: yes, 3 minutes initial incubation at 95 °C Cycling speed: fast or ultra-fast Detection type: Probe-based Reference dye: none Multiplex: up to 5 plexes simultaneously Compatible real-time instruments: The mix is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler!
Mix Components: SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 16.5 mM MgCl2 (1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR |